Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, HPLC, Flow Cyt
Preparation and Storage
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
Images
-
Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Immobilized ab220587 at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) with a linear range of 0.016-0.5 μg/mL.
-
SDS-PAGE - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)Human CD47 (Fc Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. -
Flow Cytometry - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)FACS assay shows that ab220587 can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL.
-
Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified) (ab220587): Biotinylated Human SIRP alpha, Fc,Avitag (ab246052) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL
-
Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)The purity of Human CD47 (Fc Tag) (HPLC-verified) was greater than 90% as determined by SEC-HPLC. -
Biological activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)Loaded Recombinant human CD47 protein (Fc Chimera Active) (ab220587) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay
-
Biological activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=8.39 μg/mL
-
HPLC - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)The purity of ab220587 was greater than 90% as determined by SEC-HPLC.
