Recombinant human CD47 protein (Active) (ab174029)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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ELISA - Recombinant human CD47 protein (Active) (ab174029)
Immobilized ab221235 at 5 μg/mL (100 μL/well) binds ab174029.
Linear range: 2-13 ng/mL (QC tested).
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ELISA - Recombinant human CD47 protein (Active) (ab174029)Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) binds ab174029.
Linear range: 0.4-3 ng/mL (Routinely tested).
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Functional Studies - Recombinant human CD47 protein (Active) (ab174029)Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface can bind Human CD47 (His Tag) with an affinity constant of 1.66 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Functional Studies - Recombinant human CD47 protein (Active) (ab174029)Immobilized Human SIRP alpha (Fc Tag) (HPLC-verified) at 5 µg/mL (100 µL/well) can bind Human CD47 (His Tag) with a linear range of 6-50 ng/mL (QC tested). -
Functional Studies - Recombinant human CD47 protein (Active) (ab174029)Anti-Human CD47 MAb (Human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, binds ab174029.
Affinity constant: 1.66 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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SDS-PAGE - Recombinant human CD47 protein (Active) (ab174029)Reduced ab174029 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein > 95%
The protein migrates as 35-45 kDa under reducing condition due to glycosylation.
