Recombinant human CD47 protein (Active) (ab220594)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 10% Trehalose, 0.08% Bicine
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant human CD47 protein (Active) (ab220594)
Immobilized Human SIRP alpha, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 2-13 ng/ml.
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SDS-PAGE - Recombinant human CD47 protein (Active) (ab220594)SDS-PAGE analysis of reduced ab220594 stained overnight with Coomassie Blue.
The protein migrates as 33-44 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human CD47 protein (Active) (ab220594)Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 0.05-2.0 ng/ml.
