Recombinant human CD47 protein (Fc Chimera Active) (ab220559)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, Flow Cyt, ELISA
Preparation and Storage
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
Images
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ELISA - Recombinant human CD47 protein (Fc Chimera Active) (ab220559)
Immobilized Recombinant Human SIRP alpha protein (ab174006) at 2 μg/mL (100 μL/well) can bind Recombinant human CD47 protein (ab220559) with a linear range of 1-31 ng/mL.
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Biological activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220559)FACS analysis shows that the binding of Recombinant human CD47 protein (ab220559) to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of ab220559 used is 10 μg/mL. IC50=8.39 μg/mL.
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ELISA - Recombinant human CD47 protein (Fc Chimera Active) (ab220559)SDS-PAGE of reduced ab220559 stained overnight with Coomassie Blue. The protein migrates as 55-66 kDa due to glycosylation.
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Biological activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220559)FACS assay shows that Recombinant human CD47 protein (ab220559) can bind to ACHN cell expressing human SIRP-a. The concentration of ab220559 used is 10 μg/mL.
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Biological activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220559)Loaded Recombinant human CD47 protein (ab220559) on Protein A Biosensor, can bind Recombinant Human SIRP alpha protein (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay.
