Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Human

Overview

  • Product name

    Anti-eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 antibody [EPR9470] - BSA and Azide free
    See all eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9470] to eEF1A1/EF-Tu+eEF1A1 + eEF1AL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt (Intra), WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and 293T cell lysates. MCF-7 cells. Human brain tissue, Human breast carcinoma tissue. GST tagged Recombinant Human EEF1A1 and EEF1AL3 protein

  • General notes

    ab186380 is the carrier-free version of ab140632.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Western blot - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    All lanes : Anti-eEF1A1/EF-Tu antibody [EPR9470] (ab140632) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab140632, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Immunocytochemistry - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    This data was developed using ab140632, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling eEF1A1/EF-Tu with purified ab140632 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue). Control: PBS only.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    This data was developed using ab140632, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human brain tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    This data was developed using ab140632, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling eEF1A1/EF-Tu with ab140632 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Flow Cytometry - Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    This data was developed using ab140632, the same antibody clone in a different buffer formulation.Overlay histogram showing MCF7 cells stained with ab140632 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140632, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)
    Anti-eEF1A1/EF-Tu antibody [EPR9470] - BSA and Azide free (ab186380)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.