Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24320-27] to METTL1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free
    See all METTL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24320-27] to METTL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICCmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, NIH/3T3, PC-12, Human kidney, pancreas, HepG2, Mouse brain, testis, Rat brain and testis, RAW 264.7 lysates. IHC-P: Human pancreas and Mouse liver, Rat cerebrum tissues. ICC: HepG2, HeLa cells. Flow Cyt: HeLa, RAW 264.7 cells.

  • General notes

    ab280958 is the carrier-free version of ab271063. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    All lanes : Anti-METTL1 antibody [EPR24320-27] (ab271063) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP secondary antibody (HRP))

    Predicted band size: 31 kDa
    Observed band size: 31,28 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab271063, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 29983320, PMID: 31520064).

    Exposure time: 81 seconds

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling METTL1 with ab271063 at 1/5000 dilution (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human pancreas. The section was incubated with ab271063 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Immunocytochemistry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labelling METTL1 with ab271063 at 1/50 dilution (9.68 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear staining in HeLa cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Flow Cytometry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling METTL1 with ab271063 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    All lanes : Anti-METTL1 antibody [EPR24320-27] (ab271063) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 31 kDa
    Observed band size: 31,28 kDa why is the actual band size different from the predicted?



    This data was developed using ab271063, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 29983320, PMID: 31520064).

    Exposure time: 26 seconds

     

  • Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Western blot - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    All lanes : Anti-METTL1 antibody [EPR24320-27] (ab271063) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat testis tissue lysate
    Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 31 kDa
    Observed band size: 31,28 kDa why is the actual band size different from the predicted?



    This data was developed using ab271063, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 29983320, PMID: 31520064).

    Exposure time: Lane 1: 15 seconds; Lanes 2-5:26 seconds; Lane 6: 15 seconds.

     

  • Immunocytochemistry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Immunocytochemistry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labelling METTL1 with ab271063 at 1/50 dilution (9.68 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mainly nuclear staining in HepG2 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling METTL1 with ab271063 at 1/5000 dilution (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse liver. The section was incubated with ab271063 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling METTL1 with ab271063 at 1/5000 dilution (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on rat cerebrum. The section was incubated with ab271063 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Flow Cytometry - Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

    This data was developed using ab271063, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling METTL1 with ab271063 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)
    Anti-METTL1 antibody [EPR24320-27] - BSA and Azide free (ab280958)

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