Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-WDR4 antibody [EPR11052] (ab169526)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11052] to WDR4
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-WDR4 antibody [EPR11052]
See all WDR4 primary antibodies
Description

Rabbit monoclonal [EPR11052] to WDR4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human WDR4.
Positive control
- HepG2, HeLa, A549 and MCF7 cell lysates, Human cervical carcinoma and Human tonsil tissues, HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11052
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling ADRA1B with ab169523 at 1/500 (1.242 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on mouse stomach. The section was incubated with ab169523 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-WDR4 antibody [EPR11052] (ab169526)

All lanes : Anti-WDR4 antibody [EPR11052] (ab169526) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : WDR4 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 45 kDa
Observed band size: 49 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab169526 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

ab169526 Anti-WDR4 antibody [EPR11052] was shown to specifically react with WDR4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265109 (knockout cell lysate ab258285) was used. Wild-type and WDR4 knockout samples were subjected to SDS-PAGE. ab169526 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-WDR4 antibody [EPR11052] (ab169526)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling WDR4 with purified ab169526 at 1/2000. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

ab169526 showing +ve staining in Human colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-WDR4 antibody [EPR11052] (ab169526)

All lanes : Anti-WDR4 antibody [EPR11052] (ab169526) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

ab169526 showing +ve staining in Human hepatocellular carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

ab169526 showing +ve staining in Human ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

ab169526 showing +ve staining in Human normal pancreas tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling WDR4 with ab169526 antibody at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR4 antibody [EPR11052] (ab169526)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling WDR4 with ab169526 antibody at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-WDR4 antibody [EPR11052] (ab169526)

Immunofluorescent analysis of HeLa cells labeling WDR4 with ab169526 at 1/50.
Flow Cytometry - Anti-WDR4 antibody [EPR11052] (ab169526)

Flow Cytometrical analysis of permeabilized HeLa cells with ab169526 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
Anti-WDR4 antibody [EPR11052] (ab169526)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR4 antibody [EPR11052] (ab169526)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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Anti-WDR4 antibody (ab241297)