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Anti-KCNQ1 antibody [N37A/10] (ab84819)

Key features and details

  • Mouse monoclonal [N37A/10] to KCNQ1
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human, Chinese hamster
  • Isotype: IgG1

Overview

  • Product name

    Anti-KCNQ1 antibody [N37A/10]
    See all KCNQ1 primary antibodies

  • Description

    Mouse monoclonal [N37A/10] to KCNQ1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    Chinese hamster
    See all applications and species data

  • Immunogen

    Fusion protein: AAASSPPRAE RKRWGWGRLP GARRGSAGLA KKCPFSLELA EGGPAGGALY APIAPGAPGP APPASPAAPA APPVASDLGP RPPVSLDPRV SIYSTRRPVL, corresponding to amino acids 2-101 of Human KCNQ1 (accession number: P51787)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Lysate from transfected COS-1 cells transiently expressing KCNQ1.

  • General notes

    The clone number has been updated from S37A-10 to N37A/10, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1/1000 dilution + Human Cell lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP for 1 hour at RT

    Predicted band size: 75 kDa

  • Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Overlay histogram showing HEK293 cells stained with ab84819 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84819, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    All lanes : Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from CHO -T cells transfected with mink-KvLQT1

    Predicted band size: 75 kDa

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