Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KCNIP4 antibody [EPR18314] (ab203831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18314] to KCNIP4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KCNIP4 antibody [EPR18314]
    See all KCNIP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18314] to KCNIP4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain lysate; Mouse and rat brain lysates; Rat and mouse cerebellum lysates; Human cerebellum and midbrain lysates. IP: Mouse brain whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Rat cerebellum lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human midbrain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab203831 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831)

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