Anti-THIK-1 antibody (ab237624)
Key features and details
- Rabbit polyclonal to THIK-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-THIK-1 antibody -
Description
Rabbit polyclonal to THIK-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human THIK-1 aa 250 to the C-terminus.
Database link: Q9HB14 -
Positive control
- WB: LO2 and HepG2 whole cell lysate. IHC-P: Human skeletal muscle and glioma tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-THIK-1 antibody (ab237624)All lanes : Anti-THIK-1 antibody (ab237624) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : LO2 (human immortal liver cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 45 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THIK-1 antibody (ab237624)Paraffin-embedded human skeletal muscle tissue stained for THIK-1 with ab237624 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THIK-1 antibody (ab237624)Paraffin-embedded human glioma tissue stained for THIK-1 with ab237624 at a 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-THIK-1 antibody (ab237624)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for THIK-1 using ab237624 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).