Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EAG1 antibody (ab101174)

Key features and details

  • Rabbit polyclonal to EAG1
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-EAG1 antibody
    See all EAG1 primary antibodies

  • Description

    Rabbit polyclonal to EAG1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Rat Hippocampus; Rat Whole Brain; Mouse Hippocampus; Mouse Whole Brain.

  • General notes

    Previously labelled as KCNH1.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Rat
All applications
Cow
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).

Target

  • Function

    Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.

  • Tissue specificity

    Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells.

  • Sequence similarities

    Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily.
    Contains 1 cyclic nucleotide-binding domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.

  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EAG antibody
    • EAG channel 1 antibody
    • EAG1 antibody
    • Ether a go go potassium channel 1 antibody
    • Ether a go go, Drosophila, homolog of antibody
    • Ether-a-go-go potassium channel 1 antibody
    • ether-a-go-go, Drosophila, homolog of antibody
    • h eag antibody
    • h-eag antibody
    • hEAG1 antibody
    • Kcnh1 antibody
    • KCNH1_HUMAN antibody
    • Kv10.1 antibody
    • M eag antibody
    • MGC124419 antibody
    • MGC124420 antibody
    • MGC142269 antibody
    • Potassium voltage gated channel subfamily H (eag related), member 1 antibody
    • Potassium voltage gated channel subfamily H member 1 antibody
    • Potassium voltage-gated channel subfamily H member 1 antibody
    • Voltage gated potassium channel subunit Kv10.1 antibody
    • Voltage-gated potassium channel subunit Kv10.1 antibody
    see all

Images

  • Western blot - Anti-EAG1 antibody (ab101174)
    Western blot - Anti-EAG1 antibody (ab101174)
    All lanes : Anti-EAG1 antibody (ab101174) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

References (0)

Publishing research using ab101174? Please let us know so that we can cite the reference in this datasheet.

ab101174 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-EAG1 antibody (ab101174)
    Western blot - Anti-EAG1 antibody (ab101174)
    All lanes : Anti-EAG1 antibody (ab101174) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 108 kDa
    Observed band size: 108 kDa
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

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