Anti-KCNQ2 antibody (ab22897)
Key features and details
- Rabbit polyclonal to KCNQ2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KCNQ2 antibody -
Description
Rabbit polyclonal to KCNQ2 -
Host species
Rabbit -
Specificity
NB: Rabbit polyclonal to KCNQ2 (ab22897) was used in reducing but not in denaturing conditions in WB (i.e. not heated or boiled). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human KCNQ2.
Read Abcam's proprietary immunogen policy (Peptide available as ab25449.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Western blot - Anti-KCNQ2 antibody (ab22897) This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceAll lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/ml
Lane 1 : Rat whole brain lysate
Lane 2 : Mouse whole brain lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 95 kDaRabbit polyclonal to KCNQ2 (ab22897) was used in reducing conditions in WB but not in denaturing conditions (i.e. not heated).
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Immunocytochemistry/ Immunofluorescence - Anti-KCNQ2 antibody (ab22897) This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceImmunfluorescent detection of KCNQ2 (green colour) in differentiated murine neural precursors using Rabbit polyclonal to KCNQ2 (ab22897). KCNQ2 labeling is seen in the tips of neurite extensions (such as the cell in the top left corner), along axons, dendrites and possibily spines. ab22897 (3.5µg/ml) was incubated overnight at 4°C. Secondary antibody used is goat anti-rabbit Alexa fluor 488 (1h at RT ). The red colour is MAP2 detection (Secondary used is goat anti-mouse Alexa fluor 568; 1h at RT). NB: The white centres are caused by high intensity to-pro-3 blue nuclear counterstain cross-colouring with the red and background green.
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Western blot - Anti-KCNQ2 antibody (ab22897)All lanes : Anti-KCNQ2 antibody (ab22897) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 95 kDa
Additional bands at: 140 kDa, 15 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab22897 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Abcam recommends loading unheated lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
