Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K75/41] to Kv4.3/KCND3 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free
    See all Kv4.3/KCND3 primary antibodies

  • Description

    Mouse monoclonal [K75/41] to Kv4.3/KCND3 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum tissue; Rat cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab254279 is the carrier-free version of ab252538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254279 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

    This data was developed using ab252538, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the mouse cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

    This data was developed using ab252538, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the rat cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

    This data was developed using ab252538, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the mouse skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

    This data was developed using ab252538, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on the rat skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)
    Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (ab254279)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.