Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8106] to Rab3D - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Rab3D antibody [EPR8106] - BSA and Azide free
    See all Rab3D primary antibodies

  • Description

    Rabbit monoclonal [EPR8106] to Rab3D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248251 is the carrier-free version of ab128997 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248251 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    This data was developed using ab128997, the same antibody clone in a different buffer formulation.ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    Western blot - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : T.T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HT-29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab128997, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    This data was developed using ab128997, the same antibody clone in a different buffer formulation.ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    OI-RD Scanning - Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    This data was developed using ab128997, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
    Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)

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