Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARL2BP antibody [EPR15265-21] (ab188322)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15265-21] to ARL2BP
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARL2BP antibody [EPR15265-21]
    See all ARL2BP primary antibodies

  • Description

    Rabbit monoclonal [EPR15265-21] to ARL2BP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, Human fetal brain, HUVEC, A549, HepG2, Mouse brain, Mouse heart, Rat spleen, Raw264.7 and NIH/3T3 lysates. IHC-P: Human kidney and Rat liver tissues. ICC: A549 cells. Flow Cyt: A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15265-21

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ARL2BP knockout HeLa cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab188322 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188322 Anti-ARL2BP antibody [EPR15265-21] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab188322 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunocytochemistry/ Immunofluorescence - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling ARL2BP with ab188322 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counterstained with Dapi (blue).

  • Flow Cytometry - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Flow Cytometry - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Flow cytometry analysis of A549 (human lung carcinoma) cells labelling ARL2BP (red) with purified ab188322 at a dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody.

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Western blot - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    All lanes : Anti-ARL2BP antibody [EPR15265-21] (ab188322) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Raw264.7 cell lysate
    Lane 5 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunoprecipitation - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Western blot analysis of ARL2BP in HepG2 cell lysate immunoprecipitated with ab188322 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HepG2 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL2BP antibody [EPR15265-21] (ab188322)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ARL2BP with ab188322 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ARL2BP antibody [EPR15265-21] (ab188322)
    Anti-ARL2BP antibody [EPR15265-21] (ab188322)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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