Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)
![Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)](https://www.abcam.com/ps/products/250/ab250269/Images/ab250269-405730-anti-rab13-antibody-epr14109-b-bsa-and-azide-free-western-blot.jpg "Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14109(B)] to RAB13 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free
See all RAB13 primary antibodies -
Description
Rabbit monoclonal [EPR14109(B)] to RAB13 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250269 is the carrier-free version of ab180936. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250269 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14109(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)All lanes : Anti-RAB13 antibody [EPR14109(B)] (ab180936) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : RAB13 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Hek293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 23 kDaThis data was developed using ab180936, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab180936 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab180936 was shown to specifically react with RAB13 in wild-type HAP1 cells as signal was lost in RAB13 knockout cells. Wild-type and RAB13 knockout samples were subjected to SDS-PAGE. ab180936 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)](https://www.abcam.com/ps/products/250/ab250269/Images/ab250269-403479-anti-rab13-antibody-epr14109-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)All lanes : Anti-RAB13 antibody [EPR14109(B)] (ab180936) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Lane 2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 10 µg
Predicted band size: 23 kDaThis data was developed using ab180936, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)](https://www.abcam.com/ps/products/250/ab250269/Images/ab250269-1-anti-rab13-antibody-epr14109-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)This data was developed using ab180936, the same antibody clone in a different buffer formulation.Lysate from HeLa cells was immunoprecipitated with ab180936 at a 1/70 dilution. For the subsequent blot, ab180936 used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Second land is the negative control. -
![Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)](https://www.abcam.com/ps/products/250/ab250269/Images/ab250269-4-benefits-of-recombinant-antibodies.png)
Anti-RAB13 antibody [EPR14109(B)] - BSA and Azide free (ab250269)