Anti-ARL13B antibody [N295B/66] (ab136648)
![Anti-ARL13B antibody [N295B/66] (ab136648)](https://www.abcam.com/ps/products/136/ab136648/Images/ab136648-151212-anti-arl13b-antibody-n295b-66-immunohistochemistry.jpg "Anti-ARL13B antibody [N295B/66] (ab136648)")
Key features and details
- Mouse monoclonal [N295B/66] to ARL13B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-ARL13B antibody [N295B/66]
See all ARL13B primary antibodies -
Description
Mouse monoclonal [N295B/66] to ARL13B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Mouse
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Immunogen
corresponding to ARL13B.
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Positive control
- WB: mouse fetus tissue lysate, MEF1 whole cell lysate. IHC-P: rat normal brain (choroid plexus) FFPE tissue.
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General notes
Western blot protocol advice:
For blocking, we recommend using 1% milk for 1 hour.This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N295B/66 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL13B antibody [N295B/66] (ab136648)
IHC image of ARL13B staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136648, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-ARL13B antibody [N295B/66] (ab136648)](https://www.abcam.com/ps/products/136/ab136648/Images/ab136648-151213-anti-arl13b-antibody-n295b-66-western-blot.jpg)
Western blot - Anti-ARL13B antibody [N295B/66] (ab136648)All lanes : Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Mouse Fetus (14 Day Old) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDaThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 1 hour 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
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![Western blot - Anti-ARL13B antibody [N295B/66] (ab136648)](https://www.abcam.com/ps/products/136/ab136648/Images/ab136648-151214-anti-arl13b-antibody-n295b-66-western-blot.jpg)
Western blot - Anti-ARL13B antibody [N295B/66] (ab136648)Anti-ARL13B antibody [N295B/66] (ab136648) at 5 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 25 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab136648 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Arl13b protein has been shown to run at approximately 60-kDa. This is due to post-translational modifications (PMID:17488627).
