Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Epsin 1 antibody [EPR3023] (ab75879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3023] to Epsin 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Epsin 1 antibody [EPR3023]
    See all Epsin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3023] to Epsin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Epsin 1 aa 450-550. The exact sequence is proprietary.
    Database link: Q9Y6I3

  • Positive control

    • ICC/IF: SH-SY5Y cells WB: SH-SY5Y and HeLa cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3023

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

    Lane 1 : 293T cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 94 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

    Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.

  • Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)
    All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa

  • Anti-Epsin 1 antibody [EPR3023] (ab75879)
    Anti-Epsin 1 antibody [EPR3023] (ab75879)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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