Antibodies, Proteins, Kits and Reagents for Life Science

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100 µl 318 288 ₸ 10 µl 130 665 ₸

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Anti-Epsin 1 antibody [EPR3023] (ab75879)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3023] to Epsin 1
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Epsin 1 antibody [EPR3023]
See all Epsin 1 primary antibodies
Description

Rabbit monoclonal [EPR3023] to Epsin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Epsin 1 aa 450-550. The exact sequence is proprietary.
Database link: Q9Y6I3
Positive control
- ICC/IF: SH-SY5Y cells WB: SH-SY5Y and HeLa cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3023
Isotype

IgG
Research areas

Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + rat brain at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/1000 dilution (purified)

Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/5000 dilution (purified) + mouse brain lysate at 20 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

Immunofluorescence staining of HeLa cells with purified ab75879 at a working dilution of 1/50, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab75879 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunocytochemistry/ Immunofluorescence - Anti-Epsin 1 antibody [EPR3023] (ab75879)

Unpurified ab75879, at a 1/100 dilution, staining Epsin 1 in SH-SY5Y cells by Immunofluorescence.
Western blot - Anti-Epsin 1 antibody [EPR3023] (ab75879)

All lanes : Anti-Epsin 1 antibody [EPR3023] (ab75879) at 1/2000 dilution (unpurified)

Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 58 kDa
Anti-Epsin 1 antibody [EPR3023] (ab75879)

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