Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric) (ab273337)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Protein Disulfide Isomerase (PDI) Activity Assay Kit (Fluorometric) (ab273337) is a simple, sensitive, and high-throughput adaptable kit that utilizes the reductase activity of PDI for determining its activity in a variety of samples.
This kit employs a synthetic quenched fluorescent substrate which gets cleaved in the presence of PDI producing an enhanced fluorometric signal (Ex/Em = 490/580 nm). In the presence of a specific inhibitor, the quenched fluorescent substrate cannot be cleaved by PDI. Detection limit as low as 0.1 mU. -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Fluorescence Standard (25 µM) 1 x 200µl PDI Activator (100 mM) 1 x 40µl PDI Assay Buffer 1 x 25ml PDI Inhibitor (50X) 1 x 100µl PDI Positive Control Lyophilized 1 vial PDI Substrate 1 x 200µl
Images
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Example data
Example data.
Standard Curve.
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Example dataExample data.
Measurement of PDI activity in mouse liver lysate, Jurkat and HeLa cell extract.
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Example dataExample data.
Relative PDI Activity was calculated in Jurkat cell extract (9.2 µg), HeLa cell extract (9.1 µg) and liver lysates prepared from mice liver (4.6 µg).
