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Cancer Tumor immunology Cytokines

Anti-IL-23R antibody [EPR22838-4] (ab222104)

Price and availability

314 937 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-IL-23R antibody [EPR22838-4] (ab222104)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22838-4] to IL-23R
  • Suitable for: ICC/IF, Flow Cyt, ELISA
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-IL-23R antibody [EPR22838-4]
    See all IL-23R primary antibodies
  • Description

    Rabbit monoclonal [EPR22838-4] to IL-23R
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HEK-293T cells. Flow Cyt: HEK-293T cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22838-4
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Immunology
    • Immunoglobulins
    • Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs

Images

  • Flow Cytometry - Anti-IL-23R antibody [EPR22838-4] (ab222104)
    Flow Cytometry - Anti-IL-23R antibody [EPR22838-4] (ab222104)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged IL-23R expression vector cells labeling IL-23R with ab222104 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab225248 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc(-)(Left) and myc(+)(Right) population respectively.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] (ab222104)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] (ab222104)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-23R with ab222104 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged IL-23R expression vector is observed. Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • ELISA - Anti-IL-23R antibody [EPR22838-4] (ab222104)
    ELISA - Anti-IL-23R antibody [EPR22838-4] (ab222104)
    ELISA analysis of Human IL23R recombinant protein at 1000 ng/mL with ab222104. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-IL-23R antibody [EPR22838-4] (ab222104)
    Anti-IL-23R antibody [EPR22838-4] (ab222104)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IL-23R antibody [EPR22838-4] (ab222104)

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    Applications: WB

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    Applications: ELISA, Flow Cyt, ICC/IF

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