Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)
![Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)](https://www.abcam.com/ps/products/255/ab255964/Images/ab255964-1-anti-il-23r-antibody-epr22838-flowcytometry-293t-human.jpg "Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22838-4] to IL-23R - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, ELISA
- Reacts with: Human
Overview
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Product name
Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free
See all IL-23R primary antibodies -
Description
Rabbit monoclonal [EPR22838-4] to IL-23R - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells. Flow Cyt: HEK-293T cells.
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General notes
Ab255964 is the carrier-free version of ab222104. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255964 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22838-4 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)
Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged IL-23R expression vector cells labeling IL-23R with ab222104 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab225248 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc(-)(Left) and myc(+)(Right) population respectively.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222104).
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)](https://www.abcam.com/ps/products/255/ab255964/Images/ab255964-2-anti-il-23r-antibody-epr22838-immunocytochemistry-293t-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-23R with ab222104 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged IL-23R expression vector is observed. Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222104).
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![ELISA - Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)](https://www.abcam.com/ps/products/255/ab255964/Images/ab255964-3-anti-il-23r-antibody-epr22838-4-elisa-human-il23r.jpg)
ELISA - Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)This data was developed using ab222104, the same antibody clone in a different buffer formulation.
ELISA analysis of Human IL23R recombinant protein at 1000 ng/mL with ab222104. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody. -
![Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)](https://www.abcam.com/ps/products/255/ab255964/Images/ab255964-4-benefits-of-recombinant-antibodies.png)
Anti-IL-23R antibody [EPR22838-4] - BSA and Azide free (ab255964)
