Anti-ARL2BP antibody [EPR15265] (ab185964)
![Anti-ARL2BP antibody [EPR15265] (ab185964)](https://www.abcam.com/ps/products/185/ab185964/Images/ab185964-410127-anti-arl2bp-antibody-epr15265-western-blot-human-knockout-lysate.jpg "Anti-ARL2BP antibody [EPR15265] (ab185964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15265] to ARL2BP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ARL2BP antibody [EPR15265]
See all ARL2BP primary antibodies -
Description
Rabbit monoclonal [EPR15265] to ARL2BP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF7, Human fetal brain, HUVEC, A549 and HepG2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15265 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ARL2BP knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.
ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)](https://www.abcam.com/ps/products/185/ab185964/Images/ab185964-218918-anti-arl2bp-antibody-epr15265-western-blot.jpg)
Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HUVEC lysate
Lane 3 : A549 lysate
Lane 4 : HepG2 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 19 kDa
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![Anti-ARL2BP antibody [EPR15265] (ab185964)](https://www.abcam.com/ps/products/185/ab185964/Images/ab185964-3-benefits-of-recombinant-antibodies.png)
Anti-ARL2BP antibody [EPR15265] (ab185964)
