Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ARL2BP antibody [EPR15265] (ab185964)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15265] to ARL2BP
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-ARL2BP antibody [EPR15265]
See all ARL2BP primary antibodies
Description

Rabbit monoclonal [EPR15265] to ARL2BP
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, MCF7, Human fetal brain, HUVEC, A549 and HepG2 lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15265
Isotype

IgG
Research areas

Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)

All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ARL2BP knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 19 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab185964 observed at 20 kDa. Red - loading control ab8245 observed at 36 kDa.

ab185964 Anti-ARL2BP antibody [EPR15265] was shown to specifically react with ARL2BP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265269 (knockout cell lysate ab258312) was used. Wild-type and ARL2BP knockout samples were subjected to SDS-PAGE. ab185964 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ARL2BP antibody [EPR15265] (ab185964)

All lanes : Anti-ARL2BP antibody [EPR15265] (ab185964) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HUVEC lysate
Lane 3 : A549 lysate
Lane 4 : HepG2 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa
Anti-ARL2BP antibody [EPR15265] (ab185964)

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