Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2752] to Alpha B Crystallin - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free
    See all Alpha B Crystallin primary antibodies

  • Description

    Rabbit monoclonal [EPR2752] to Alpha B Crystallin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247476 is the carrier-free version of ab76467 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247476 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Rat eyeball lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Alpha B Crystallin with purified ab76467 at 1/250. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human brain with purified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/1000 dilution (purified) + Human fetal heart tissue lysate at 10 µg/ml

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?



    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    Immunofluorescence staining of HeLa cells with purified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
  • Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

    ab76467 (purified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    This data was developed using ab76467, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human brain with unpurified ab76467 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    This data was developed using ab76467, the same antibody clone in a different buffer formulation.Immunohistochemical staining of alpha B Crystallin in paraffin embedded human heart muscle with ab76467 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Western blot - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Lane 1 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/5000 dilution
    Lane 2 : Anti-Alpha B Crystallin antibody [EPR2752] (ab76467) at 1/20000 dilution

    Lane 1 : Fetal heart tissue lysate
    Lane 2 : Rat eyeball tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    This data was developed using ab76467, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunocytochemistry - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    This data was developed using ab76467, the same antibody clone in a different buffer formulation.Immunofluorescence staining of HeLa cells with unpurified ab76467 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
  • Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Immunoprecipitation - Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    This data was developed using ab76467, the same antibody clone in a different buffer formulation.ab76467 (unpurified) at 1/250 immunoprecipitating alpha B crystallin in human fetal heart (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
  • Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)
    Anti-Alpha B Crystallin antibody [EPR2752] - BSA and Azide free (ab247476)

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