Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)
![Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)](https://www.abcam.com/ps/products/76/ab76535/Images/ab76535-92007-anti-rac1-cdc42-phospho-s71-antibody-ep221y-western-blot.jpg "Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y]
See all RAC1 + Cdc42 primary antibodies -
Description
Rabbit monoclonal [EP221Y] to RAC1 + Cdc42 (phospho S71) -
Host species
Rabbit -
Specificity
This antibody only detects RAC1 + Cdc42 phosphorylated on Serine 71. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A synthetic phospho peptide corresponding to residues surrounding Serine 71 of human RAC1 + Cdc42
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Positive control
- WB: A431 cells treated with Lambda Phosphatase.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP221Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)All lanes : Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535) at 1/1000 dilution
Lane 1 : A431 cell lysate treated with Lambda Phosphatase
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 24 kDa why is the actual band size different from the predicted?
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![Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)](https://www.abcam.com/ps/products/76/ab76535/Images/ab76535-3-benefits-of-recombinant-antibodies.png)
Anti-RAC1 + Cdc42 (phospho S71) antibody [EP221Y] (ab76535)
