Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
Key features and details
- Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAC1 + Cdc42 (phospho S71) antibody
See all RAC1 + Cdc42 primary antibodies -
Description
Rabbit polyclonal to RAC1 + Cdc42 (phospho S71) -
Host species
Rabbit -
Specificity
Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho peptide (Human) containing serine 71. The sequence is conserved in human and mouse RAC 1, 2, and 3, and Cdc 42 human, mouse, rat, dog and frog.
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Positive control
- A431 cells treated with EGF.
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General notes
RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RAC 1. The final product is generated by affinity chromatography using a RAC 1 derived peptide that is phosphorylated at serine 71. -
Primary antibody notes
RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)Peptide Competition and Phosphatase Treatment:
Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5482 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the peptide corresponding to ab5482 blocks the antibody signal. The data also shows that phosphatase stripping eliminates the signal, verifying that the antibody is pho
