Anti-TFII I (phospho Y248) antibody (ab63344)
Key features and details
- Rabbit polyclonal to TFII I (phospho Y248)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFII I (phospho Y248) antibody
See all TFII I primary antibodies -
Description
Rabbit polyclonal to TFII I (phospho Y248) -
Host species
Rabbit -
Specificity
ab63344 detects endogenous levels of TFII I only when phosphorylated at tyrosine 248. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
synthesized phosphopeptide derived from human TFII I around the phosphorylation site of tyrosine 248 (P-D-YP-Y-Q).
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Positive control
- Extracts from LOVO cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TFII I (phospho Y248) antibody (ab63344)All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution
Lane 1 : extracts from LOVO cells, without immunising peptide
Lane 2 : extracts from LOVO cells, with immunising peptide
Predicted band size: 112 kDa
Observed band size: 112 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
