Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TFII I antibody [EPR7697] (ab129025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7697] to TFII I
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TFII I antibody [EPR7697]
    See all TFII I primary antibodies

  • Description

    Rabbit monoclonal [EPR7697] to TFII I

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TFII I aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, U937, and Jurkat lysates; Human colon tissue

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TFII I antibody [EPR7697] (ab129025)
    Western blot - Anti-TFII I antibody [EPR7697] (ab129025)
    All lanes : Anti-TFII I antibody [EPR7697] (ab129025) at 1/10000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : U937 cell lysates
    Lane 4 : Jurkat cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 112 kDa
    Observed band size: 135-138 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7697] (ab129025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7697] (ab129025)

    ab129025, at 1/100 dilution staining TFII I in formalin fixed paraffin embedded Human colon tissue by immunohistochemistry

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TFII I antibody [EPR7697] (ab129025)
    Immunocytochemistry/ Immunofluorescence - Anti-TFII I antibody [EPR7697] (ab129025)
    ab129025, at 1/100 dilution, staining TFII I in Hela cells by immunofluorescence
  • OI-RD Scanning - Anti-TFII I antibody [EPR7697] (ab129025)
    OI-RD Scanning - Anti-TFII I antibody [EPR7697] (ab129025)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TFII I antibody [EPR7697] (ab129025)
    Anti-TFII I antibody [EPR7697] (ab129025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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