Anti-TFII I antibody [EPR7696] (ab134133)
![Anti-TFII I antibody [EPR7696] (ab134133)](https://www.abcam.com/ps/products/134/ab134133/Images/TFII-I-Primary-antibodies-ab134133-4.jpg "Anti-TFII I antibody [EPR7696] (ab134133)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7696] to TFII I
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TFII I antibody [EPR7696]
See all TFII I primary antibodies -
Description
Rabbit monoclonal [EPR7696] to TFII I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human TFII I aa 50-150. The exact sequence is proprietary.
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Positive control
- Human fetal brain lysates; Jurkat, U937, HeLa and 293T cell lysates; Human colon and kidney tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Dissociation constant (KD)
KD = 1.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7696 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TFII I antibody [EPR7696] (ab134133)All lanes : Anti-TFII I antibody [EPR7696] (ab134133) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 112 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)](https://www.abcam.com/ps/products/134/ab134133/Images/TFII-I-Primary-antibodies-ab134133-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TFII I with ab134133 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)](https://www.abcam.com/ps/products/134/ab134133/Images/TFII-I-Primary-antibodies-ab134133-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFII I antibody [EPR7696] (ab134133)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TFII I with ab134133 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-TFII I antibody [EPR7696] (ab134133)](https://www.abcam.com/ps/products/134/ab134133/Images/TFII-I-Primary-antibodies-ab134133-5.jpg)
OI-RD Scanning - Anti-TFII I antibody [EPR7696] (ab134133) -
![Anti-TFII I antibody [EPR7696] (ab134133)](https://www.abcam.com/ps/products/134/ab134133/Images/ab134133-5-benefits-of-recombinant-antibodies.png)
Anti-TFII I antibody [EPR7696] (ab134133)
