Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HOXB13 antibody [EPR17371] (ab201682)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17371] to HOXB13
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-HOXB13 antibody [EPR17371]
See all HOXB13 primary antibodies
Description

Rabbit monoclonal [EPR17371] to HOXB13
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human prostate cancer lysate; LNCaP and PC-3 cell lysates. IHC-P: Human carcinoma of prostate tissue. IP: LNCaP cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17371
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody [EPR17371] (ab201682)

Immunohistochemical analysis of paraffin-embedded Human carcinoma of prostate tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human carcinoma of prostate tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-HOXB13 antibody [EPR17371] (ab201682)

All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution

Lane 1 : Human prostate cancer lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
HOXB13 is predominantly expressed in the prostate.
Immunoprecipitation - Anti-HOXB13 antibody [EPR17371] (ab201682)

ab201682 (purified) at 1/20 dilution immunoprecipitating HOXB13 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 µg.
Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab201682 & LnCaP whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab201682 in LnCaP whole cell lysate

For western blotting, ab201682 at 1/500 dilution (0.15 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.
Blocking and diluting buffer: 5% NFDM /TBST.
Western blot - Anti-HOXB13 antibody [EPR17371] (ab201682)

All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution

Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
HOXB13 is predominantly expressed in the prostate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody [EPR17371] (ab201682)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human transitional cell carcinoma of bladder represents a HOXB13 negative tissue. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody [EPR17371] (ab201682)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cerebral cortex represents a HOXB13 negative tissue. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-HOXB13 antibody [EPR17371] (ab201682)

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