Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Tyrosinase antibody [EPR10141] (ab170905)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10141] to Tyrosinase
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Tyrosinase antibody [EPR10141]
See all Tyrosinase primary antibodies
Description

Rabbit monoclonal [EPR10141] to Tyrosinase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human Tyrosinase. The exact sequence is proprietary.
Database link: P14679
Positive control
- Human melanoma lysate, Human melanoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10141
Isotype

IgG
Research areas

Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)

Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution + Human melanoma lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] (ab170905)

ab170905 staining Tyrosinase in A375 (human malignant melanoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control: PBS only.
Flow Cytometry - Anti-Tyrosinase antibody [EPR10141] (ab170905)

Flow cytometry analysis of A375 (human malignant melanoma) cells labeling with purified ab170905 at 1/30 dilution ( 10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) (1/2000 dilution) was used as the secondary antibody. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue, labeling Tyrosinase using ab170905 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Tyrosinase antibody [EPR10141] (ab170905)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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