Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10141] to Tyrosinase - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free
    See all Tyrosinase primary antibodies

  • Description

    Rabbit monoclonal [EPR10141] to Tyrosinase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Tyrosinase. The exact sequence is proprietary.
    Database link: P14679

  • General notes

    Ab240163 is the carrier-free version of ab170905. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240163 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)
    Flow Cytometry - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)

    Flow cytometry analysis of A375 (human malignant melanoma) cells labeling with purified ab170905 at 1/30 dilution ( 10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) (1/2000 dilution) was used as the secondary antibody. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

     

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170905).

  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)
    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)

    ab170905 staining Tyrosinase in A375 (human malignant melanoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170905).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)

    Immunohistochemical analysis of paraffin-embedded Human melanoma tissue, labeling Tyrosinase using ab170905 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170905).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)
    Anti-Tyrosinase antibody [EPR10141] - BSA and Azide free (ab240163)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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