Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PC4 antibody [EPR9281] (ab154852)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9281] to PC4
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PC4 antibody [EPR9281]
    See all PC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR9281] to PC4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PC4 aa 1-100. The exact sequence is proprietary.
    Database link: P53999

  • Positive control

    • MCF7, T47D, Jurkat and PC3 cell lysates; MCF7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9281

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
    Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
    All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution (purified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47-D cell lysate
    Lane 3 : PC-3 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
    Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

    Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)
    Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)
    ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 μg Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
    Western blot - Anti-PC4 antibody [EPR9281] (ab154852)
    All lanes : Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : T47D cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)
    Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

    Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.

  • Anti-PC4 antibody [EPR9281] (ab154852)
    Anti-PC4 antibody [EPR9281] (ab154852)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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