Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Delta-catenin antibody [EPR17628] (ab184917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17628] to Delta-catenin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Delta-catenin antibody [EPR17628]
    See all Delta-catenin primary antibodies

  • Description

    Rabbit monoclonal [EPR17628] to Delta-catenin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, hippocampus and cerebellum; and rat brain and hippocampus tissue lysates. PC-12 whole cell lysate. ICC/IF: Neuro-2a cells.

  • General notes

     

     

     This product was previously labelled as delta 2 Catenin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (ab184917)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

     

  • Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 10 seconds

  • Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Rat hippocampus lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 3 minutes

  • Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917)
    All lanes : Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

    Lane 1 : Mouse hippocampus lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time = 10 seconds

  • Anti-Delta-catenin antibody [EPR17628] (ab184917)
    Anti-Delta-catenin antibody [EPR17628] (ab184917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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