Anti-CD161 antibody [EP7169] (ab137059)
Key features and details
- Rabbit monoclonal [EP7169] to CD161
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD161 antibody [EP7169]
See all CD161 primary antibodies -
Description
Rabbit monoclonal [EP7169] to CD161 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to residues on the C terminal of Human CD161 (UniProt ID: Q12918).
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Positive control
- WB: Human small intestine tissue lysate, HuT-78, THP1, and Caco 2 cell lysates. Flow Cyt: HuT-78 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7169 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of HuT-78 cells labelling CD161 with ab137059 at 1/10 dilution (red) or a Rabbit IgG negative control (green). FITC-conugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/150 dilution.
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All lanes : Anti-CD161 antibody [EP7169] (ab137059) at 1/1000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?