Anti-GREB1 antibody (ab72999)
Key features and details
- Rabbit polyclonal to GREB1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GREB1 antibody
See all GREB1 primary antibodies -
Description
Rabbit polyclonal to GREB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human GREB1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab92716) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF (1) Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).IHC-P Use a concentration of 1 µg/ml.Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).IHC-P
Use a concentration of 1 µg/ml.Target
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Function
Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers. -
Tissue specificity
Expressed in proliferating prostatic tissue and prostate cancer. -
Sequence similarities
Belongs to the GREB1 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9687 Human
- Omim: 611736 Human
- SwissProt: Q4ZG55 Human
- Unigene: 467733 Human
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Alternative names
- 5730583K22Rik antibody
- 9130004E13 antibody
- AF180470 antibody
see all
Images
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Western blot - Anti-GREB1 antibody (ab72999)Anti-GREB1 antibody (ab72999) at 1 µg/ml + Human Prostate Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 216 kDa
Observed band size: 216 kDa
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab72999)ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab72999)IHC image of GREB1 antibody staining in a section of formalin-fixed paraffin-embedded normal human prostate performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab72999, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab72999 has been referenced in 13 publications.
- Duijndam B et al. Physiologically Relevant Estrogen Receptor Alpha Pathway Reporters for Single-Cell Imaging-Based Carcinogenic Hazard Assessment of Estrogenic Compounds. Toxicol Sci 181:187-198 (2021). PubMed: 33769548
- Tashjian RZ et al. The effect of estrogen-like compound on rotator cuff tendon healing in a murine model. J Orthop Res N/A:N/A (2021). PubMed: 33533088
- Kochi M et al. Oncogenic mutation in RAS-RAF axis leads to increased expression of GREB1, resulting in tumor proliferation in colorectal cancer. Cancer Sci 111:3540-3549 (2020). PubMed: 32629543
- Haines CN et al. GREB1 regulates PI3K/Akt signaling to control hormone-sensitive breast cancer proliferation. Carcinogenesis 41:1660-1670 (2020). PubMed: 32894276
- Yang Y et al. MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression. Mol Ther Nucleic Acids 22:846-861 (2020). PubMed: 33230480
Images
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Western blot - Anti-GREB1 antibody (ab72999)Anti-GREB1 antibody (ab72999) at 1 µg/ml + Human Prostate Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 216 kDa
Observed band size: 216 kDa
Exposure time: 2 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab72999)ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab72999)
IHC image of GREB1 antibody staining in a section of formalin-fixed paraffin-embedded normal human prostate performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab72999, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.