Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16725] to AKR1C3 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free
    See all AKR1C3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16725] to AKR1C3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251405 is the carrier-free version of ab203834. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251405 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/5000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 10 seconds


    This data was developed using ab203834, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution + MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    This data was developed using ab203834, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Western blot - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    All lanes : Anti-AKR1C3 antibody [EPR16725] (ab203834) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 3 minutes


    This data was developed using ab203834, the same antibody clone in a different buffer formulation.

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM /TBST or 1% BSA/TBST.

  • Immunoprecipitation - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Immunoprecipitation - Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    This data was developed using ab203834, the same antibody clone in a different buffer formulation.AKR1C3 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab203834 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203834 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Lane 1: Human fetal liver whole cell lysate 10ug (Input). Lane 2: ab203834 IP in Human fetal liver whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203834 in Human fetal liver whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds.
  • Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)
    Anti-AKR1C3 antibody [EPR16725] - BSA and Azide free (ab251405)

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