Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Friday March 05, 2021

Anti-Prealbumin antibody [2E10C5] (ab204997)

Key features and details

Mouse monoclonal [2E10C5] to Prealbumin
Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
Reacts with: Human
Isotype: IgG1

Product name

Anti-Prealbumin antibody [2E10C5]
See all Prealbumin primary antibodies
Description

Mouse monoclonal [2E10C5] to Prealbumin
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Prealbumin aa 1 to the C-terminus. Expressed in E coli.
Database link: P02766

Run BLAST with Run BLAST with
Positive control
- Human Prealbumin (aa1-147) recombinant protein, Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate, A549 or MCF-7 cells, Human liver cancer tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2E10C5
Isotype

IgG1
Research areas

Western blot - Anti-Prealbumin antibody [2E10C5] (ab204997)

All lanes : Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution

Lane 1 : HEK293 whole cell lysate
Lane 2 : Prealbumin (aa1-147)-hIgGFc transfected HEK293 whole cell lysate

Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prealbumin antibody [2E10C5] (ab204997)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded section) analysis of Human liver cancer tissue labeling Prealbumin with ab204997 at a 1/200 dilution. DAB staining used.
Flow Cytometry - Anti-Prealbumin antibody [2E10C5] (ab204997)

Flow cytometric analysis of A549 cells labelling Prealbumin with ab204997 (green) at a 1/200 dilution and negative control (red).
Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [2E10C5] (ab204997)

Immunofluorescence analysis of MCF-7 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor^® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Prealbumin antibody [2E10C5] (ab204997)

Immunofluorescence analysis of A549 cells labeling Prealbumin with ab204997 at a 1/200 dilution (green), followed by Alexa Fluor^® 555 phalloidin (red). Nuclear counterstain DRAQ5 fluorescent DNA dye used (blue).
Western blot - Anti-Prealbumin antibody [2E10C5] (ab204997)

Anti-Prealbumin antibody [2E10C5] (ab204997) at 1/500 dilution + Human Prealbumin (aa1-147) recombinant protein

Predicted band size: 16 kDa
Additional bands at: 45.8 kDa. We are unsure as to the identity of these extra bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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