Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)]
    See all Glucose Transporter GLUT4 primary antibodies

  • Description

    Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal heart, mouse heart, rat heart

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution

    Lane 1 : Human fetal heart whole cell lysates
    Lane 2 : Human fetal heart whole cell lysates with Phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Dot Blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

    Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.

    5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Western blot - Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution

    Lane 1 : Untreated human fetal heart
    Lane 2 : Lambda phosphorylase treated human fetal heart

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)
    Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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