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Citrate Synthase Activity Assay Kit (ab119692)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 3 hr 15 min
  • Sample type: Cell culture extracts, Tissue Extracts
  • Sensitivity: 1.56 µg/ml

Overview

  • Product name

    Citrate Synthase Activity Assay Kit
    See all Citrate synthetase kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 3 5.45%
    Inter-assay
    Sample n Mean SD CV%
    1 3 8.3%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Enzyme activity

  • Sensitivity

    1.56 µg/ml

  • Range

    1.56 µg/ml - 100 µg/ml

  • Assay time

    3h 15m

  • Species reactivity

    Reacts with: Cow, Human
    Does not react with: Mouse, Rat

  • Product overview

    Citrate Synthase Activity Assay Kit (ab119692) is used to determine mitochondrial citrate synthase (CS) activity in a sample. The enzyme is immunocaptured within the wells of a microplate, and activity is determined by recording color development of TNB, which is generated in the reaction of citrate synthesis. The reaction product, TNB, absorbs at OD = 412 nm.


    This kit immunocaptures only native citrate synthase from the samples. Using the immunocapture method allows the measurement of citrate synthase activity with simple sample preparation and no need for mitochondrial isolation.


    This kit can be used with whole cell extracts or tissue homogenates.


    Citrate synthase assay protocol summary:
    - add samples to wells
    - incubate for 3 hrs to capture enzyme
    - wash with wash buffer
    - add activity solution
    - analyze with microplate reader for 5-30 min at 20s intervals

  • Notes

    Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 2.3.3.1). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Storage: All components are shipped cold. 10X Reaction Reagent mixture is shipped lyophilized. Before use, rehydrate the reaction mixture by adding 1mL base buffer. Vortex the tube thoroughly to dissolve the lyophilized powder to prepare 10X Reaction solution. After hydration unused amounts of the 10X Reaction solution should be stored at -80°C for 6 months. Store all other components at 4°C. This kit is stable for 6 months from receipt.

  • Platform

    Microplate reader

Properties

Images

  • ELISA - Citrate Synthase Activity Assay Kit (ab119692)
    ELISA - Citrate Synthase Activity Assay Kit (ab119692)
    Figure 2. Example activity measurements from titrated of Human heart homogenate.
  • ELISA - Citrate Synthase Activity Assay Kit (ab119692)
    ELISA - Citrate Synthase Activity Assay Kit (ab119692)
    Figure 1. Example activity measurements from titrated HepG2 cells.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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