Citrate Synthase Assay Kit (ab239712)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture media, Cell culture supernatant, Tissue
Overview
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Product name
Citrate Synthase Assay Kit
See all Citrate synthetase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Tissue, Cell culture media -
Product overview
Citrate Synthase Assay Kit (ab239712) provides reagents for the measurement of citrate synthase activity in various tissues/cells. Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product. The rate of color development is proportional to the enzyme activity. The assay is simple, rapid and can detect Citrate Synthase activity less than 1 mU in a variety of samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CS Assay Buffer 1 x 25ml CS Developer (Lyophilized) 1 vial CS Positive Control (Lyophilized) 1 vial CS Substrate Mix (Lyophilized) 1 vial GSH Standard (reduced) (Lyophilized) 1 vial -
Research areas
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Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
Sequence similarities
Belongs to the citrate synthase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- CISY_HUMAN
- Citrate synthase
- Citrate synthase, mitochondrial
see all
Images
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GSH Standard Curve
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Citrate Synthase Activity in Jurkat cell lysate (10 µg), rat liver lysate (20 µg), purified yeast mitochondria (4 µg) and CS Positive Control (2 µL).
