Anti-PGAM4 antibody [OTI2A12] (ab279384)
![Anti-PGAM4 antibody [OTI2A12] (ab279384)](https://www.abcam.com/ps/products/279/ab279384/Images/ab279384-2-AntiPGAM4-antibody-OTI2A12-westernblot-HEK293T-MCF7-cells.jpg "Anti-PGAM4 antibody [OTI2A12] (ab279384)")
Key features and details
- Mouse monoclonal [OTI2A12] to PGAM4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PGAM4 antibody [OTI2A12] -
Description
Mouse monoclonal [OTI2A12] to PGAM4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PGAM4 aa 1-254. NP_001025062
Database link: Q8N0Y7 -
Positive control
- WB: HEK-293T, MCF7 cell extract. IHC-P: Human embryonic cerebellum, human adult heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
OTI2A12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PGAM4 antibody [OTI2A12] (ab279384)All lanes : Anti-PGAM4 antibody [OTI2A12] (ab279384) at 1/500 dilution
Lane 1 : HEK-293T cell extract
Lane 2 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 antibody [OTI2A12] (ab279384)](https://www.abcam.com/ps/products/279/ab279384/Images/ab279384-4-AntiPGAM4-antibody-OTI2A12-immunohistochemistry-embryonic-cerebellum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 antibody [OTI2A12] (ab279384)Paraffin embedded human embryonic cerebellum stained for PGAM4 using ab279384 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 antibody [OTI2A12] (ab279384)](https://www.abcam.com/ps/products/279/ab279384/Images/ab279384-3-AntiPGAM4-antibody-OTI2A12-immunohistochemistry-adult-heart-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM4 antibody [OTI2A12] (ab279384)Paraffin embedded human adult heart tissue stained for PGAM4 using ab279384 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.
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![Western blot - Anti-PGAM4 antibody [OTI2A12] (ab279384)](https://www.abcam.com/ps/products/279/ab279384/Images/ab279384-1-AntiPGAM4-antibody-OTI2A12-westernblot-HEK293T-cells.jpg)
Western blot - Anti-PGAM4 antibody [OTI2A12] (ab279384)All lanes : Anti-PGAM4 antibody [OTI2A12] (ab279384) at 1/2000 dilution
Lane 1 : HEK-293T cells transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected pCMV6-ENTRY PGAM4 cDNA for 48 hrs and lysed.
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa