Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Serum, Tissue Lysate
- Sensitivity: 0.001 mU/well
Overview
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Product name
Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric)
See all AKR1A1 kits -
Detection method
Colorimetric -
Sample type
Serum, Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.001 mU/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric) (ab211112) provides a convenient tool for sensitive detection of Aldo-Keto Reductase (AKR) activity in animal tissue and cells, as well as biological fluids such as serum. The assay is based on the ability of AKR to reduce a general substrate and convert NADP+ to NADPH, which reacts with the AKR probe to generates color that can be detected at OD 450 nm. The intensity of the color is proportional to the activity of AKR in the sample.
This assay can detect as low as 1 µU of AKR activity in samples. It has been validated with AKR1B10, AKR1C1 and AKR1C3.
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Notes
Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members and are present in nearly all phyla. They are mainly monomeric soluble proteins (34 – 37 kD) and their common is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification.
In humans, AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AKR Assay Buffer 1 x 25ml AKR Substrate 1 x 15ml AKR Probe 1 vial AKR Positive Control 1 x 10µl NADPH Standard 1 vial -
Research areas
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Function
Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). -
Tissue specificity
Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung. -
Sequence similarities
Belongs to the aldo/keto reductase family. - Information by UniProt
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Alternative names
- AK1A1_HUMAN
- Akr1a1
- Alcohol dehydrogenase
see all
Images
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AKR Activity Assay Kit (Colorimetric) (ab211112)
Typical NADPH standard curve.
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AKR Activity Assay Kit (Colorimetric) (ab211112)Kinetic curves showing AKR activity detection in increase amounts of positive control included in the kit (μL/assay).
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AKR Activity Assay Kit (Colorimetric) (ab211112)AKR specific activity in human tissue lysates and cell line lysates. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve.