Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-NOL5A antibody [CL2603]
See all NOL5A primary antibodies
Description

Mouse monoclonal [CL2603] to NOL5A
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human NOL5A aa 450-550.
Database link: O00567

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence FSKEELMSSDLEETA as determined by overlapping synthetic peptides.
Positive control
- IHC-P: Human prostate cancer, fallopian tube, kidney, liver, breast cancer and cervix tissue. WB: U-251 MG cell lysate. ICC/IF: HeLa, A431, MCF7, U-2 OS and U-251 MG cells.
General notes

This product was previously labelled as NOL5A

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL2603
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (ab242201)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling NOL5A using ab242201 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-NOL5A antibody [CL2603] (ab242201)

All lanes : Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/ml

Lane 1 : Non-transfected U-251 MG (Human brain glioma cell line) cell lysate
Lane 2 : siRNA#1 transfected U-251 MG cell lysate
Lane 3 : siRNA#2 transfected U-251 MG cell lysate

Predicted band size: 66 kDa

Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (ab242201)

Immunocytochemistry/Immunofluorescence analysis of U-251 MG (Human brain glioma cell line) cells labeling NOL5A using ab242201 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (ab242201)

Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling NOL5A using ab242201 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human kidney tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (ab242201)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling NOL5A using ab242201 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human liver tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-NOL5A antibody [CL2603] (ab242201)

Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma cell line) cells labeling NOL5A using ab242201 at 4 µg/ml (green). Nuclear probe is visualized in blue, microtubules in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-NOL5A antibody [CL2603] (ab242201)

Anti-NOL5A antibody [CL2603] (ab242201) at 1 µg/ml + U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody [CL2603] (ab242201)

Formalin-fixed, paraffin-embedded human cervix tissue stained for NOL5A with ab242201 at 1/500 dilution in immunohistochemical analysis.