Anti-PRAF1 antibody (ab88623)
Key features and details
- Mouse polyclonal to PRAF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAF1 antibody
See all PRAF1 primary antibodies -
Description
Mouse polyclonal to PRAF1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length Human PRAF1 isoform 2 Swiss-Prot Q9GZS1 (NP_071935.1)
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Positive control
- U-2 OS cell lysate, PRAF1 transfected 293T cell lysate and HeLa cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRAF1 antibody (ab88623)Anti-PRAF1 antibody (ab88623) at 1 µg/ml + U-2 OS at 50 µg
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-PRAF1 antibody (ab88623)All lanes : Anti-PRAF1 antibody (ab88623) at 1 µg/ml
Lane 1 : PRAF1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-PRAF1 antibody (ab88623)Immunofluorescence of ab88623 on HeLa cell (antibody concentration 10 ug/ml)
