Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K56A/50] to CASK - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CASK antibody [K56A/50] - BSA and Azide free
    See all CASK primary antibodies

  • Description

    Mouse monoclonal [K56A/50] to CASK - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain lysate; mouse brain lysate; rat brain lysate; mouse cerebellum lysate; rat cerebellum lysate. IHC-P: Rat liver, stomach and pancreas tissue; mouse liver tissue.

  • General notes

    ab255764 is the carrier-free version of ab252540.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Western blot - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    All lanes : Anti-CASK antibody [K56A/50] (ab252540) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse cerebellum lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Anti-mouse IgG for IP (HRP) (ab131368) at 1/100000 dilution
    Lanes 2-5 : Peroxidase-Conjugated Goat anti-Mouse IgG(H+L) at 1/100000 dilution

    Predicted band size: 105 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 125 seconds; Lanes 2-5: 26 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 15236336).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous and cytoplasmic staining on rat stomach is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat pancreas is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on rat liver is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CASK with ab252540 at 0.134µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Membranous staining on mouse liver is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252540 for 30 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab252540).

  • Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)
    Anti-CASK antibody [K56A/50] - BSA and Azide free (ab255764)

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