Anti-COPS3/CSN3 antibody (ab229807)
Key features and details
- Rabbit polyclonal to COPS3/CSN3
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COPS3/CSN3 antibody
See all COPS3/CSN3 primary antibodies -
Description
Rabbit polyclonal to COPS3/CSN3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus monkey, Orangutan, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human COPS3/CSN3 aa 150 to the C-terminus.
Database link: Q9UNS2-1 -
Positive control
- WB: MCF7, HeLa, HEK-293T and HT-29 whole cell lysates; Mouse heart, brain and ovarian lysates. IHC-P: Human testis and heart tissues. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COPS3/CSN3 antibody (ab229807)All lanes : Anti-COPS3/CSN3 antibody (ab229807) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse brain lysate
Lane 7 : Mouse ovarian lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (ab229807)Paraffin-embedded human testis tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (ab229807)Paraffin-embedded human heart tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-COPS3/CSN3 antibody (ab229807)COPS3/CSN3 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab229807.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab229807 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg.
For western blotting, an HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.
