Recombinant human JAK1 protein (D966-989) (ab268686)
Key features and details
- Expression system: Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant human JAK1 protein (D966-989)
See all JAK1 proteins and peptides -
Biological activity
JAK1 activity assay was performed with and without IRS-1 (Y608). The recombinant mutant (JAK1) did not show significant kinase activity in vitro.
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Purity
> 70 % SDS-PAGE. -
Expression system
Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
NGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQ VQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNIS FMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGE HLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFE AASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLT TPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKL SDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEIC YNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPF FRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFG KVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENI VKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQI CKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTV KDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMAL FLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPS NRTSFQNLIEGFEALLK -
Predicted molecular weight
133 kDa -
Actual molecular weight
105 kDa -
Amino acids
438 to 1154 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant human JAK1 (aa966-aa989 deleted).
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Description
Recombinant human JAK1 protein (Active)
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human JAK1 protein (D966-989) (ab268686)
Recombinant Human JAK1 (d966-989) protein purity determined using SDS-PAGE analysis.
MW ~105 kDa.
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Functional Studies - Recombinant human JAK1 protein (D966-989) (ab268686)Recombinant Human Jak1 (d966-989) protein activity with and without IRS-1 (Y608). The recombinant mutant did not show significant kinase activity in vitro.