Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)
Key features and details
- Rabbit polyclonal to ERK1 (phospho Y204) + ERK2 (phospho Y187)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody -
Description
Rabbit polyclonal to ERK1 (phospho Y204) + ERK2 (phospho Y187) -
Host species
Rabbit -
Specificity
This antibody is specific for ERK1, only when phosphorylated at tyrosine 204 -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
A synthesized phosphopeptide derived around the phosphorylation site of ERK1 tyrosine 204 and ERK2 tyrosine 187 (T-E-YP-V-A).
-
Positive control
- Extracts from Jurkat cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with PMA (200ng/ml, 15min)
Lane 2 : Extracts from Jurkat cells un-treated
Predicted band size: 44, 42 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted?
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)Ab47339 staining human normal or colon tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract treated with PMA
Predicted band size: 44, 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
