Protein Aggregation Assay Kit (ab234048)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Protein Aggregation Assay Kit -
Detection method
Fluorescent -
Product overview
Protein Aggregation Assay Kit (ab234048) is a platform to estimate the extent of aggregation within a protein/antibody sample. This is a fast, high-throughput, plate-based fluorometric assay that can detect as low as 0.5% antibody aggregate.
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Notes
Antibody and protein aggregation are important issues in the field of Biotherapeutics. A wide range of conditions and different stages of processing of biopharmaceuticals, such as, formulation, purification, storage, shipping and handling, heat, freeze thaw cycles, mechanical stress, chemical treatment etc. might cause aggregation of proteins, enzymes and antibody targets. The possibility of protein aggregates in eliciting immunogenicity is a major concern for biotherapeutics, which might compromise the safety and efficacy of the biologically derived drug products. Recombinant protein production and storage would highly benefit from a high throughput screening method that can screen a variety of formulation and storage conditions of a particular target protein/antibody. Currently, different biophysical techniques such as Dynamic Light Scattering (DLS), Size-exclusion Chromatography with Multi-angle Light Scattering (SEC-MALS) can detect the presence of small amount of aggregates in a protein/antibody preparation, but they are not amenable for high-throughput screening format.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Aggregation Assay Buffer 1 x 12ml Aggregation Assay Probe 1 x 20µl Antibody aggregate standard (0%) 1 x 1500µl Antibody aggregate standard (10%) 1 x 1500µl
Images
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Size exclusion chromotography of protein aggregates.
Size Exclusion Chromatography of 100% non-aggregated antibody and 100% aggregated antibody samples are premixed in appropriate ratio and provided as 0-10% Antibody Aggregate Standards.
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Aggregation Standard plot% Aggregation Standard Plot using the Antibody Aggregate Standards (with 5 mg/mL, final amount: 500 μg/well.
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Aggregation Standard plot% Aggregation Standard Plot obtained using the Antibody Aggregate Standards (1 mg/mL, final amount: 100 μg/well).
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Protein Aggregation under various storage conditions.Protein Aggregation detected after a proprietary protein sample (10 mg/mL) was subjected to different formulation and storage conditions.
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Detected protein Aggreagtion in various refolding buffers.Protein Aggregation detected after refolding of denatured lysozymes using different buffer formulations.