Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18663] to NAT10 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NAT10 antibody [EPR18663] - BSA and Azide free
    See all NAT10 primary antibodies

  • Description

    Rabbit monoclonal [EPR18663] to NAT10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251186 is the carrier-free version of ab194297 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251186 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ALP.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunocytochemistry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red). The negative controls are as follows:-
    -ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Western blot - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Western blot - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 116 kDa
    Observed band size: 116 kDa


    Exposure time: 30 seconds


    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on rat colon tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Flow Cytometry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NAT10 (red) with purified ab194297 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Western blot - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Western blot - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 116 kDa
    Observed band size: 116 kDa


    Exposure time: 30 seconds


    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunocytochemistry - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red). The negative controls are as follows:-
    -ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on mouse stomach tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Immunoprecipitation - Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

    This data was developed using ab194297, the same antibody clone in a different buffer formulation.

    NAT10 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate with ab194297 at 1/80 dilution. Lane 1: HeLa cell lysate 10ug (Input). Lane 2: ab194297 IP in HeLa cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194297 in HeLa cell lysate. Western blot was performed from the immunoprecipitate using ab194297 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
  • Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
    Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)

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